This function will calculate the relative amount of a fraction compared to
the whole source using either qPCR copies or DNA concentrations. This works
by summing all of the within a source, and then dividing each fraction
by the total. The total percent will always equal 1 (or 100%) when running
this function.
Usage
add_gradient_pos_rel_amt(
data,
amt,
source_mat_id = "source_mat_id",
overwrite = FALSE
)
Arguments
- data
(dataframe) Sample metadata
- amt
(string) Column name that has the qPCR or DNA amounts per fraction
- source_mat_id
(string, default: "source_mat_id") Column name with the source_mat_id
- overwrite
(bool, default: FALSE) Determines whether or not to overwrite an existing gradient_pos_rel_amt column
Value
A dataframe with a gradient_pos_rel_amt
column
Details
There may be times that you would want to provide values that do not sum to
100%. For example, if you throw out a certain fraction because it didn't
sequence well, then your total could be less than 100%.