For qSIP2, the choices are limited to one of 12C, 13C, 14N, 15N, 16O
or 18O
If you’re sample is unfractionated, you can also use “bulk”,
“unfractionated” or “T0”
This term is also a required MISIP field
isotopolog: The name or ID of the labeled substrate
This term is also a required MISIP field
For qSIP2 it doesn’t have to strictly adhere to MISIP standards, and
a string such as “glucose” will suffice
timepoint: The timepoint of the experiment. The unit
doesn’t matter, but it should be consistent across the dataset.
Designating the timepoint is required for growth calculations
total_abundance: The total copy number in the
unfractionated source material. This is required for growth
calculations
volume: The volume of DNA that the
total_abundance was measured in. Can just be set to
1 if all samples have the same volume. This is required for
growth calculations.
Sample Data
sample_id: Unique name for the sample
This term is also used as the link between sample metadata and
source metadata
gradient_position: The numerical position of the sample
in the gradient. Informally refered to as “fraction”.
gradient_pos_density: The density of the gradient at
this position
gradient_pos_amt: The total mass, concentration or copy
numbers at the position
gradient_pos_rel_amt: The relative amount of the
gradient_pos_amt compared to the total amount of the source
material
Feature Data
feature_id: A unique ID for each feature
feature type: Explains what the values in the feature
table represent. For example “counts”, “relative”, etc.
